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KMID : 0861020080230040091
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Korea Journal of Herbology
2008 Volume.23 No. 4 p.91 ~ p.101
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The Effects of Ampelopsis Radix on Allergic Inflammation in PMA-stimulated Human Mast Cells
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Kim Jang-Hyun
Cheon Jin-Hong
Kim Sung-Yun
Park Yong-Ki
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Abstract
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Objectives: In this study, we investigated the inhibitory effects of Ampelopsis Radix methanol(AR-M) extract on allergic inflammation in activated human mast cells and its potential therapeutic or toxic effects.
Methods: Ampelopsis Radix(AR) was extracted with 80% methanol. HMC-1 cells, a human mast cell line, were treated with different concentrations of AR-M extract, and then stimulated with PMA plus A23187. The cell toxicity of AR-M extract was determined by MTT assay. The concentrations of and cytokines were measured by ELISA. The gene expression of COX-2 and its protein levels were determined by RT-PCR and Western blot. The phosphorylation of ERK MAPK and the NF- activation were determined by Western blot.
Results: AR-M extract was significantly inhibited the production of PGE2 and inflammatory cytokines(TNF-, IL-6 and IL-8) in PMA/A23187-stimulated HMC-1 cells. AR-M extract also attenuated the mRNA expression of COX-2 and its protein induction. Furthermore, AR-M extract attenuated PMA/A23187-induced phophorylation of ERK1/2 MAPK and the NF- p65 subunit translocation into nuclear of HMC-1 cells. AR-M extract significantly decreased PMN A23187-induced release of histamine in a dose-dependent manner.
Conclusions: These results indicate that Ampelopsis Radix shows the property of anti-allergic inflammation In vitro through suppressing the production of inflammatory mediators released from mast cells, suggesting have a potential for the treatment of allergic diseases.
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KEYWORD
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Ampelopsis Radix, allergic inflammation, mast cells, NF- pathway
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